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mssa strain s aureus atcc 29213  (ATCC)


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    ATCC mssa strain s aureus atcc 29213
    Mssa Strain S Aureus Atcc 29213, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9738 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Purification:

    Article Title: Ceanothane and lupane type triterpenes from Zizyphus joazeiro - an anti-staphylococcal evaluation.
    Article Snippet: The present paper describes the phytochemical and anti-staphylococcal activity investigation of the dichloromethane extract of the Brazilian plant Zizyphus joazeiro Mart.. The purification steps were guided by bioassays against 17 bacterial strains of clinical sources, including methicillinresistant (MRSA) and ‐sensitive (MSSA) Staphylococcus aureus as well as MRSA (ATCC 33591) and MSSA (ATCC 29213) reference strains.. One of the more active fractions is comprised of three lupane-type triterpenes, the methylbetulinate (1) as well as the known betulinic (2) and alphitolic (3) acids and, for the first time in the Z. joazeiro, two ceanothane type triterpenes, the methylceanothate (4) and the epigouanic acid A (5).



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    mssa strain s aureus atcc 29213  (ATCC)
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    A. MRSA expresses five PBPs, PBP1, PBP2, PBP2A (pink), PBP3, and PBP4. B. FICI values calculated based on MRSA USA300 grown in the presence of BAY 11-7082 and another antibiotic. FICI values <0.5 represent synergy, values between 0.5-4 represent no interaction, and values >4 represent antagonism. C. FICI values for MRSA USA300 (pink) and MSSA <t>ATCC</t> <t>29213</t> (blue) grown in the presence of BAY 11-7082 and a β-lactam that synergizes with BAY 11-7082 in MRSA. Data represent an average of three independent experiments performed in triplicate.
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    reference mssa strain  (ATCC)
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    Synthesis and in vitro evaluation of 18 F-glucopyranosyl- d -sorbitol analogs. (A) Schematic of the synthesis of 18 F-glucopyranosyl- d -sorbitol analogs from [ 18 F]­FDG via reverse phosphorolysis and subsequent reduction. (B) Chemical structures of 18 F-glucopyranosyl- d -sorbitol analogs used in this study, which differ by glycosidic linkages and stereochemistry. The highlighted portions of analogs correspond to the [ 18 F]­FDS scaffold. RCY: radiochemical yield (nondecay corrected), RCP: radiochemical purity. (C,D) In vitro uptake of [ 18 F]­FNT, [ 18 F]­FMT, [ 18 F]­FLT, [ 18 F]­FCT, and [ 18 F]­FDS (control) by the indicated Gram-positive (C) and Gram-negative (D) bacterial pathogens. (E) Specificity of [ 18 F]­FNT uptake in S. aureus (Ctrl, ATCC 29213) reflected by reduced uptake in the presence of [ 19 F]­FNT (1 mM, blocking) and in heat killed bacteria. (F) Differential uptake of [ 18 F]­FNT in methicillin-resistant S. <t>aureus</t> <t>(MRSA)</t> and methicillin-sensitive S. aureus <t>(MSSA)</t> clinical isolates compared to reference strain ( S. aureus ATCC 29213; MSSA). **** P < 0.0001 by unpaired Student’s t -test.
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    bacterial strains methicillinsensitive staphylococcus aureus atcc 29213 mssa  (ATCC)
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    Synthesis and in vitro evaluation of 18 F-glucopyranosyl- d -sorbitol analogs. (A) Schematic of the synthesis of 18 F-glucopyranosyl- d -sorbitol analogs from [ 18 F]­FDG via reverse phosphorolysis and subsequent reduction. (B) Chemical structures of 18 F-glucopyranosyl- d -sorbitol analogs used in this study, which differ by glycosidic linkages and stereochemistry. The highlighted portions of analogs correspond to the [ 18 F]­FDS scaffold. RCY: radiochemical yield (nondecay corrected), RCP: radiochemical purity. (C,D) In vitro uptake of [ 18 F]­FNT, [ 18 F]­FMT, [ 18 F]­FLT, [ 18 F]­FCT, and [ 18 F]­FDS (control) by the indicated Gram-positive (C) and Gram-negative (D) bacterial pathogens. (E) Specificity of [ 18 F]­FNT uptake in S. aureus (Ctrl, ATCC 29213) reflected by reduced uptake in the presence of [ 19 F]­FNT (1 mM, blocking) and in heat killed bacteria. (F) Differential uptake of [ 18 F]­FNT in methicillin-resistant S. <t>aureus</t> <t>(MRSA)</t> and methicillin-sensitive S. aureus <t>(MSSA)</t> clinical isolates compared to reference strain ( S. aureus ATCC 29213; MSSA). **** P < 0.0001 by unpaired Student’s t -test.
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    strain atcc 29213 mssa  (ATCC)
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    Synthesis and in vitro evaluation of 18 F-glucopyranosyl- d -sorbitol analogs. (A) Schematic of the synthesis of 18 F-glucopyranosyl- d -sorbitol analogs from [ 18 F]­FDG via reverse phosphorolysis and subsequent reduction. (B) Chemical structures of 18 F-glucopyranosyl- d -sorbitol analogs used in this study, which differ by glycosidic linkages and stereochemistry. The highlighted portions of analogs correspond to the [ 18 F]­FDS scaffold. RCY: radiochemical yield (nondecay corrected), RCP: radiochemical purity. (C,D) In vitro uptake of [ 18 F]­FNT, [ 18 F]­FMT, [ 18 F]­FLT, [ 18 F]­FCT, and [ 18 F]­FDS (control) by the indicated Gram-positive (C) and Gram-negative (D) bacterial pathogens. (E) Specificity of [ 18 F]­FNT uptake in S. aureus (Ctrl, ATCC 29213) reflected by reduced uptake in the presence of [ 19 F]­FNT (1 mM, blocking) and in heat killed bacteria. (F) Differential uptake of [ 18 F]­FNT in methicillin-resistant S. <t>aureus</t> <t>(MRSA)</t> and methicillin-sensitive S. aureus <t>(MSSA)</t> clinical isolates compared to reference strain ( S. aureus ATCC 29213; MSSA). **** P < 0.0001 by unpaired Student’s t -test.
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    A. MRSA expresses five PBPs, PBP1, PBP2, PBP2A (pink), PBP3, and PBP4. B. FICI values calculated based on MRSA USA300 grown in the presence of BAY 11-7082 and another antibiotic. FICI values <0.5 represent synergy, values between 0.5-4 represent no interaction, and values >4 represent antagonism. C. FICI values for MRSA USA300 (pink) and MSSA ATCC 29213 (blue) grown in the presence of BAY 11-7082 and a β-lactam that synergizes with BAY 11-7082 in MRSA. Data represent an average of three independent experiments performed in triplicate.

    Journal: bioRxiv

    Article Title: BAY 11-7082 potentiates select β-lactams to inhibit growth of methicillin-resistant Staphylococcus aureus

    doi: 10.64898/2026.01.13.699313

    Figure Lengend Snippet: A. MRSA expresses five PBPs, PBP1, PBP2, PBP2A (pink), PBP3, and PBP4. B. FICI values calculated based on MRSA USA300 grown in the presence of BAY 11-7082 and another antibiotic. FICI values <0.5 represent synergy, values between 0.5-4 represent no interaction, and values >4 represent antagonism. C. FICI values for MRSA USA300 (pink) and MSSA ATCC 29213 (blue) grown in the presence of BAY 11-7082 and a β-lactam that synergizes with BAY 11-7082 in MRSA. Data represent an average of three independent experiments performed in triplicate.

    Article Snippet: We conducted checkerboard assays with MRSA strain USA300, which has mecA ( , Figure S2 ), as well as methicillin-sensitive S. aureus (MSSA) strain ATCC 29213, which does not, to understand the potential role of PBP2a ( , Figure S3 ).

    Techniques:

    Synthesis and in vitro evaluation of 18 F-glucopyranosyl- d -sorbitol analogs. (A) Schematic of the synthesis of 18 F-glucopyranosyl- d -sorbitol analogs from [ 18 F]­FDG via reverse phosphorolysis and subsequent reduction. (B) Chemical structures of 18 F-glucopyranosyl- d -sorbitol analogs used in this study, which differ by glycosidic linkages and stereochemistry. The highlighted portions of analogs correspond to the [ 18 F]­FDS scaffold. RCY: radiochemical yield (nondecay corrected), RCP: radiochemical purity. (C,D) In vitro uptake of [ 18 F]­FNT, [ 18 F]­FMT, [ 18 F]­FLT, [ 18 F]­FCT, and [ 18 F]­FDS (control) by the indicated Gram-positive (C) and Gram-negative (D) bacterial pathogens. (E) Specificity of [ 18 F]­FNT uptake in S. aureus (Ctrl, ATCC 29213) reflected by reduced uptake in the presence of [ 19 F]­FNT (1 mM, blocking) and in heat killed bacteria. (F) Differential uptake of [ 18 F]­FNT in methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) clinical isolates compared to reference strain ( S. aureus ATCC 29213; MSSA). **** P < 0.0001 by unpaired Student’s t -test.

    Journal: JACS Au

    Article Title: Regioselective Glycosylation of Fluorine-18-Labeled Sorbitol for Enhanced Bacterial Detection In Vivo Using PET

    doi: 10.1021/jacsau.5c01153

    Figure Lengend Snippet: Synthesis and in vitro evaluation of 18 F-glucopyranosyl- d -sorbitol analogs. (A) Schematic of the synthesis of 18 F-glucopyranosyl- d -sorbitol analogs from [ 18 F]­FDG via reverse phosphorolysis and subsequent reduction. (B) Chemical structures of 18 F-glucopyranosyl- d -sorbitol analogs used in this study, which differ by glycosidic linkages and stereochemistry. The highlighted portions of analogs correspond to the [ 18 F]­FDS scaffold. RCY: radiochemical yield (nondecay corrected), RCP: radiochemical purity. (C,D) In vitro uptake of [ 18 F]­FNT, [ 18 F]­FMT, [ 18 F]­FLT, [ 18 F]­FCT, and [ 18 F]­FDS (control) by the indicated Gram-positive (C) and Gram-negative (D) bacterial pathogens. (E) Specificity of [ 18 F]­FNT uptake in S. aureus (Ctrl, ATCC 29213) reflected by reduced uptake in the presence of [ 19 F]­FNT (1 mM, blocking) and in heat killed bacteria. (F) Differential uptake of [ 18 F]­FNT in methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) clinical isolates compared to reference strain ( S. aureus ATCC 29213; MSSA). **** P < 0.0001 by unpaired Student’s t -test.

    Article Snippet: [ 18 F] FNT exhibited higher uptake in clinical MSSA and MRSA isolates compared to the reference MSSA strain ( F, S. aureus ATCC 29213, P < 0.0001).

    Techniques: In Vitro, Control, Blocking Assay, Bacteria